Cell

Part:BBa_J23015:Design

Designed by: Samantha Liang   Group: iGEM06_Berkeley   (2006-07-24)


F plasmid pOX38/MC1061 oriTf knockout,(pOX38; oriTf::CmR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ForlamF Forward oligo to knockout OriT: tttatccgtaaataatttaacccactccacaaaaaggctcaacag gtgtaggctggagctgcttc ForlamR Reverse oligo to knockout OriT: ggaccatggctaattcccat ctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaa


Source

PCR FORlamF/ForlamR on pKD3 (~1000bp, dPN1, Neb2) Electroporate into pKD46xpOX38

References